QUANTITATIVE REAL-TIME PCR (Q-PCR) FOR SPUTUM SMEAR DIAGNOSIS OF PULMONARY TUBERCULOSIS AMONG PEOPLE WITH HIV/AIDS
نویسندگان
چکیده
منابع مشابه
Quantitative Real-time Pcr (q-pcr) for Sputum Smear Diagnosis of Pulmonary Tuberculosis among People with Hiv/aids
OBJECTIVE To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV/AIDS with a clinical suspicion of PTB. METHOD This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV/AIDS with a clinical suspicion of PTB, a...
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Pulmonary tuberculosis is a common infection worldwide. In the eastern part of Iran, fluctuations in tuberculosis prevalence are seen due to the migration of people from Afghanistan. The aim of this study was to evaluate the time of bacteriologic sputum conversion after treatment and affecting factors in those whose smear does not become negative.This study was carried out on 85 smear-positive ...
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Background: Molecular detection has recently been proposed by nucleic acid amplification, known as polymerase chain reaction (PCR). The aim of this study was to compare the diagnostic method of smear and polymerase chain reaction with culture in terms of sensitivity, specificity, positive and negative predictive value in the diagnosis of pulmonary tuberculosis. Methods: In this cross-sectional...
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Karen R Steingart, Andrew Ramsay and Madhukar Pai Author for correspondence Deptartment of Epidemiology, Biostatistics & Occupational Health, McGill University, Respiratory Epidemiology & Clinical Research Unit, Montréal Chest Institute, 1020 Pine Avenue West, Montréal H3A 1A2, Canada Tel.: +1 514 398 5422 Fax: +1 514 398 4503 [email protected] Expert Rev. Anti Infect. Ther. 5(3), 327–331 ...
متن کاملQuantitative Real-Time PCR
Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 2014
ISSN: 0036-4665
DOI: 10.1590/s0036-46652014000200009